CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments

Daily CRISPR Updates on Instagram: "Integrating large DNA segments – more than one kilobase – efficiently using CRISPR-Cas9 tools is challenging. The RED-CRISPR method, which combines recombinases (Redα/β) with CRISPR-Cas9, now offers a powerful approach for precise kilobase-scale DNA insertion. This tool makes homology-directed repair – a natural way cells fix DNA by using a matching DNA template to insert new DNA accurately – 2 to 5 times more efficient. Up to 45% knock-in efficiency was achie

Tackling CRISPR-Cas9 Gene Editing Inefficiency | Bio-Radiations

Dr. Xanthine on Instagram: "what's your favorite application of CRISPR, and does it echo through the molecular gloving community? credit for footage: nature video CRISPR: Gene editing and beyond, Liquid FlowArt, zac.gloves . Park SJ, Lee GE, Cho SM, Choi EH. Recent applications, future perspectives, and limitations of the CRISPR-Cas system. Mol Ther Nucleic Acids. 2025 Jul 17;36(3):102634. "Although originally characterized as an adaptive immune system in bacteria and archaea, CRISPR-Cas9 has tr

H | Academic Coach | Medical writer on Instagram: "DNA Repair mechanisms; HDR (homology-directed repair) and NHEJ (non-homologous end joining) are two major DNA repair mechanisms for double-strand breaks. NHEJ is a fast, dominant, and error-prone pathway that directly ligates broken ends, ideal for gene knockouts. While HDR is a slower, less efficient pathway that uses a homologous template to make precise corrections, used for gene knock-ins. For more detail visit link in bio! I simply scientif